Ryan Timothy Yu
EDUCATION
College / University
University of the Philippines Diliman
Highest Degree
Bachelor of Science
Major Subjects
Molecular Biology and Biotechnology
Country
Philippines
Lab Experience
Molecular Biology: DNA/RNA/Plasmid/Protein Extraction, DNA purification, PCR, TA- and sticky-end cloning, bacterial transformation, gene splicing by overlap extension (site-directed mutagenesis), AGE, SDS-PAGE, Western Blot, Preparation of Ultra-competent cells, Flow Cytometry, AKTA Protein Purification, Ni-NTA Protein Purification, Large-scale protein expression, Avidity-based Extracellular Interaction Screening (AVEXIS), Streptavidin-AVEXIS (SAVEXIS)
Cell Biology and Imaging: Maintenance of adherent and suspension cultures, Freeze-down and revival of cells, Cell counting, Transfection via Lipofection and PEG, Cancer assays (Cell proliferation assay, Scratch wound assay, Caspase-3/7 GLO Apoptosis assay), Cytoskeletal staining, Immunocytochemistry, fluorescence microscopy, Dual-luciferase assay, Protein expression, Use of the InCell 6000 High-content Imaging System
Projects / Research
- 09/2015 – 05/2019: “Distinct Oncogenic Properties of the Novel Non-hotspot NRAS E132K mutation”. Disease Molecular Biology and Epigenetics Laboratory, National Institute of Molecular Biology and Biotechnology, University of the Philippines Diliman
- 09/2017 – 11/2017; 06/2018 – 05/2019: DDHP Project: “Confirmatory and Orthogonal Assays to Eliminate Artefactual Drug Bioactivities”, Disease Molecular Biology and Epigenetics Laboratory, National Institute of Molecular Biology and Biotechnology, University of the Philippines Diliman
- 02/2018 – 05/2018: “Resurrection of the ancestral RH5 invasion ligand provides a molecular explanation for the origin of P. falciparum malaria in humans“. Wright Group, Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Saffron Walden, UK, CB10-1SA.
Scholarships / Awards
| 2019 – 2020: | Stipend by the International Max Planck Research School |
| 2017: | Sanger Institute Prize |