Andrei Phillip David

EDUCATION

College / University

University of the Philippines Diliman

Highest Degree

Master of Science

Major Subjects

Cell and Molecular Biology

Country

Philippines

Lab Experience

Molecular biology: Cloning, PCR, site-directed mutagenesis via gene splicing by overlap extension, TA- and sticky-end ligation, bacterial transformation, plasmid preparation, preparation of ultracompetent cells, protein purification, chromatography (affinity, ion exchange, gel filtration), SDS-PAGE, western blot, GST pull-down
Cell biology and imaging: Routine maintenance of cells, transient transfection by lipofection, cellular assays (MTS assay, caspase-3/7 assay, scratch wound assay, endothelial cell tube formation assay, COX-2 luciferase assay, DCFDA staining, glucose uptake assay, phalloidin staining), light microscopy, high-content imaging

Projects / Research

2022 – 2024: Drug Discovery: “Confirmatory and orthogonal assays to eliminate artefactual drug bioactivities”. National Institute of Molecular Biology and Biotechnology, University of the Philippines Diliman, Philippines
2022 – 2024: Thesis: “Functional characterization of the novel RRAS R78W and E63D mutants identified in Filipino young-onset colorectal cancer patients”. National Institute of Molecular Biology and Biotechnology, University of the Philippines Diliman, Philippines

Scholarships / Awards

2024 – 2025:

Stipend by the International Max Planck Research School

SCIENTIFIC INTERESTS AND GOALS

My past research endeavors allowed me to investigate the role of mutations in the onset and development of diseases such as cancer. Doing so made me realize that mutations are not the sole drivers of diseases. Perturbations in gene regulation through the action of non-coding RNAs and RNA-binding proteins can also be culprits in the progression of diseases. Through this program, I would like to gain a deeper understanding of gene regulation at the structural, molecular, and cellular levels.