Jose Lorenzo FerrerEDUCATIONCollege / UniversityUniversity of the Philippines DilimanHighest DegreeBachelor of ScienceMajor SubjectsMolecular Biology and Biotechnology |
![]() CountryPhilippines |
Lab Experience
Various molecular and cellular biology techniques including gene cloning, transfection via lipofection, mammalian cell culture, cancer hallmark-associated assays including cellular proliferation assay, scratch wound assay, focus formation assay, apoptosis assay, semi-/quantitative RT-PCR, cytochemistry and immunocytochemistry, Western blotting, exosome isolation (including RNA/protein isolation from exosomes), light and fluorescence microscopy, dual-luciferase assay, site-directed mutagenesis by overlap extension PCR, gene splicing by overlap extension PCR, siRNA/shRNA and CRISPR guide RNA design, RNA secondary folding prediction, protein interaction modelling (docking, etc.).Projects / Research
- 10/2015 – 08/2016: Science research specialist: Confirmatory and orthogonal assays to eliminate artefactual drug bioactivities. Tumor-released exosomes. Detection and differential sorting of CRNDE and other CRC-associated lncRNAs. Functionalization of CRNDE lncRNA via overexpression and knockdown. Functional characterization of LUCAT1 lncRNA. Functional characterization of novel NRAS mutations
- 02/2014 – 06/2015: Undergraduate thesis student: Functional Sequelae of CRNDE lncRNA overexpression
- 04/2013 – 02/2014: Research apprentice
Scholarships / Awards
2016 – 2017: | Stipend by the International Max Planck Research School |