Luis Felipe Monge Mora
EDUCATION
College / University
Costa Rica Institute of Technology
Highest Degree
Bachelor of Science
Major Subjects
Biotechnology Engineering
Country
Costa Rica
Lab Experience
Bacterial transformation and plasmid preparation, lipofectamine-mediated transfection, protein isolation and quantification (BSA Assay), SDS-PAGE, Western Blot, immunocytochemistry, DNA isolation, PCR, agarose gel electrophoresis, RNA isolation, RT-PCR, qPCR, cell culture techniques (trypsinization, cryopreservation, thawing and cell counting). Animal-related experience: Wildtype and Cre/lox transgenic mice handling. Mouse genotyping, isolation and cryopreservation of adult hippocampus and prefrontal cortex, mice perfusion, microtome/ cryostat sectioning, DAB chromogenic staining, synaptosome preparation, spleen isolation. Preparation of primary cultures of embryonic hippocampal neurons and adult adipose tissue-derived mesenchymal stem cells, differentiation assays (osteogenic, chondrogenic and adipogenic), skin regeneration assays.
Projects / Research
- 2019 – 2020: “Analysis of murine models in vitro and in vivo for the study of Neuregulins overexpression in the Central Nervous System”. Bachelor’s thesis, supervisor: Dr. María Clara Soto Bernardini, Biotechnology Research Center (Cartago, Costa Rica) and the Max Planck Institute for Experimental Medicine (Göttingen, Germany)
- 2018 – 2019: “Profiling and validation of molecular pathophysiology downstream of NRG1-ErbB4 hyperstimulation with relevance for Schizophrenia” Dr. María Clara Soto Bernardini, Biotechnology Research Center
- 2018 – 2019: “Investigation of neuronal signaling pathways downstream of NRG2” Dr. María Clara Soto Bernardini, Biotechnology Research Center
- 2018 – 2019: “Mechanisms of NRG2-mediated adaptive signaling in GABAergic cortical networks in vivo” Dr. María Clara Soto Bernardini, Biotechnology Research Center
- 2015 – 2018: “Isolation, identification and characterization of Mesenchymal Stem Cells derived from murine adipose tissue for its incorporation in biological scaffolds and application in tissue engineering” MSc. Silvia Castro Piedra, Biotechnology Research Center
Scholarships / Awards
2021 – 2022: | Stipend by the International Max Planck Research School |
2019: | Student Mobility Program Scholarship by the Costa Rica Institute of Technology |
2017: | English Language Reinforcement Program Scholarship by the Costa Rica Institute of Technology |