Single Molecule Detection and Spectroscopy in Fluids and on Surfaces

Educated as a theoretical physicist, I am interested in a thorough theoretical understanding and modeling of all aspects of SMS experiments, including optical detection, molecule diffusion, hydrodynamics, statistics, photophysics etc. However, the main goal of my research is, on the basis of the theoretical work, to devise optimized experimental set-ups and to develop clever data evaluation algorithms for getting the most out of single-molecule spectroscopy. Especially, the application of SMS to chemical analysis, medical diagnostics, molecular biology, and cell physiology stands in the center of my efforts. A complete list of my publications can be find here. If you have any questions concerning SMS, please feel free to contact me. I am always glad to provide numerical routines for data analysis, or to find collaborators for new exciting research projects.

Single molecule detection and spectroscopy (SMS) in liquids by laser induced fluorescence is a fascinating technique with numerous applications both to fundamental research and life sciences. An excellent introduction and overview to this field can be found in the book

Zander C., Enderlein J., Keller R.A. (Editors)
Single-Molecule Detection in Solution: Methods and Applications
VCH-Wiley, 2002

A comprehensive overview of the scientific literature until ca. 1996 can be found in

Enderlein J., Ambrose W.P., Goodwin P.M., Keller R.A.
"Fluorescence detection of single molecules applicable to small volume assays"
in: Microsystem technology: A powerful tool for biomolecular studies; Eds.: M. Köhler, T. Mejevaia, H.P. Saluz (Birkhäuser, Basel, 1999) 311-29.

There is also available a special tutorial on the Electrodynamics of Single-Molecule Fluorescence (6.2 MB!).