Hari Krishnan Radhakrishnan

EDUCATION

College / University

SRM Institute of Science and Technology, Chennai

Highest Degree

Bachelor of Technology / Bachelor of Engeneering

Major Subjects

Biotechnology (Cell Biology, Tissue Engineering, Stem cell Technology, Bioprocess Engineering, Genetics, Biochemistry, Chemical Engineering)

Country

India

Lab Experience

Bacterial and Mammalian cell culture, Cryopreservation, Nucleic acid isolation, Spectrophotometry, Agarose gel electrophoresis, SDS-PAGE, c-DNA synthesis, PCR, RT-qPCR, Western Blot, Luciferase reporter assay, Bacterial transformation, Basic flow cytometry, Basic immunological assays, SEM, Bioreactor operation, Fermentation, Bacterial growth kinetics, chromatography, Nanoparticle synthesis and analysis, Liposome preparation, Bioinformatics (sequence retrieval, alignment, phylogenetic tree construction, primer designing, RNA-protein docking, network analysis, and miRNA-circRNA analysis), and basics of AutoCAD and ProE

Projects / Research

2021 – 2022: Student Researcher at Tissue Engineering and Cancer Research Lab, SRMIST. "circ_CUX1/miR-130b-5p/p300 axis in PTH stimulation of p300 expression in osteoblasts"-Bachelors Thesis. Publication: Krishnan RH, Sadu L, Das UR, Satishkumar S, Pranav Adithya S, Saranya I, Akshaya RL, Selvamurugan N. Role of p300, a histone acetyltransferase enzyme, in osteoblast differentiation. Differentiation. 2022 Mar-Apr;124:43-51.
doi: 10.1016/j.diff.2022.02.002. PMID: 35180610
2021: "Role of Wntless/Evi protein in intracellular Wnt transport", Cell and developmental Biology Lab, IISER Bhopal
2020: "Almond gum as a drug carrier- drug entrapment and drug release kinetics", Biolim Pvt Ltd., Chennai

Scholarships / Awards

2022 – 2023:	Stipend by the International Max Planck Research School
	1^st rank in the Department of Biotechnology, SRMIST, Chennai
2019, 2020, 2021:	Academic Excellence Scholarship by SRMIST, Chennai
	Best Thesis Award, Department of Biotechnology, SRMIST

SCIENTIFIC INTERESTS AND GOALS

I'm interested in exploring the field of non-coding RNAs (ncRNA), especially in diseases like cancer. Once believed as being useless, ncRNAs (miRNAs, circRNAs, lncRNAs) have been identified in almost every part of our body. ncRNAs are only recently discovered, and their structural and functional roles are yet to be fully explored. But one thing we know is that ncRNAs have the capability to regulate key signaling pathways that change the fate of the cells. What fascinates me more is using these molecules as biomarkers and therapeutic targets. My goal is to contribute in understanding these key molecules and how they can be engineered to be used as therapeutics for cancer. Nonetheless, I believe in not limiting myself to a single scientific area and would be open to exploring exciting research opportunities.